Shaping Actin Network Organization and Composition with Force

Dynamic composition, shaping and organization of plastid nucleoids

In this article recent progress on the elucidation of the dynamic composition and structure of plastid nucleoids is reviewed from a structural perspective. Plastid nucleoids are compact structures of multiple copies of different forms of ptDNA, RNA, enzymes for replication and gene expression as well as DNA binding proteins. Although early electron microscopy suggested that plastid DNA is almos...

Branching actin network remodeling governs the force-velocity relationship

Actin networks, acting as an engine pushing against an external load, are fundamentally important to cell motility. A measure of the effectiveness of an engine is the velocity the engine is able to produce at a given force, the force-velocity curve. One type of force-velocity curve, consisting of a concave region where velocity is insensitive to increasing force followed by a decrease in veloci...

Actin Remodeling Protein Cofilin Actin Network Organization through the Expression of Rotavirus NSP4 Alters the

Shaping knowledge management: organization and national culture

Purpose – The purpose of this paper is to confirm quantitatively the previous finding that organizational characteristics influence knowledge management, and to assess whether the national culture of knowledge workers equally affects the management of knowledge. Design/methodology/approach – Based on data gathered from a questionnaire survey of a Japanese pharmaceutical company’s 14 foreign sub...

Effect of Actin Organization on the Stiffness of Living Breast Cancer Cells Revealed by Peak-Force Modulation Atomic Force Microscopy.

We study the correlation between cytoskeleton organization and stiffness of three epithelial breast cancer cells lines with different degrees of malignancy: MCF-10A (healthy), MCF-7 (tumorigenic/noninvasive), and MDA-MB-231 (tumorigenic/invasive). Peak-force modulation atomic force microscopy is used for high-resolution topography and stiffness imaging of actin filaments within living cells. In...